eLife10h
A conformational fingerprint for amyloidogenic light chains
This important study identifies the "H-state" as a potential conformational marker distinguishing amyloidogenic from non-amyloidogenic light chains, addressing a critical problem in protein misfolding ...
eLife12h
The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
In this study, our results uncover the function and mechanism of CHMP5 in the regulation of cell senescence and bone formation in osteogenic cells. Deletion of Chmp5 causes endolysosomal dysfunction ...
eLife19h
Development of a new genotype–phenotype linked antibody screening system
A Golden Gate-based dual-expression vector enables rapid screening, which facilitates efficient isolation of high-affinity cross-reactive antibodies for therapeutic or diagnostic use and provides a ...
eLife1d
Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death
This study presents important findings on cold tolerance shared between hibernating and non-hibernating mammals, identifying a key molecule, GPX4, through multi-species genome-wide CRISPR screens. The ...
eLife1d
Molecular dynamics simulations illuminate the role of sequence context in the ELF3-PrD-based temperature sensing mechanism in plants
In this potentially valuable computational study, the authors conducted atomistic and coarse-grained simulations to probe the temperature-dependent phase behaviors of ELF3, a disordered component of ...
eLife1d
Proactive distractor suppression in early visual cortex
This well-written report uses functional neuroimaging in human observers to provide convincing evidence that activity in the early visual cortex is suppressed at locations that are frequently occupied ...
eLife4d
Introduction of cytosine-5 DNA methylation sensitizes cells to oxidative damage
This important work advances our understanding of DNA methylation and its consequences for susceptibility to DNA damage. This work presents evidence that DNA methylation can accentuate the genomic ...
eLife4d
TET2 contributes to gluconeogenesis and pathology of type 2 diabetes
Department of Radiation Oncology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ...
eLife9d
Transcriptional pattern enriched for synaptic signaling is associated with shorter survival of patients with high-grade serous ovarian cancer
Penn Ovarian Cancer Research Center and Basser Center for BRCA, University of Pennsylvania, Perelman School of Medicine, Philadelphia, United States ...
eLife4d
In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins
(C) Immunoprecipitation of the endogenous Squ from ovarian lysate revealed the interaction with Spn-E protein. Proteins were detected by western blotting analysis using the specific antibody for each ...
eLife8d
The promise and peril of comparing fluorescence lifetime in biology revealed by simulations
Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the measurement of absolute signal levels, independent of sensor concentration.
eLife7d
Mistargeted retinal axons form synaptically segregated subcircuits in the visual thalamus of albino mice
Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, USA ...